This Kit supports purification of circulating DNA from 1mL plasma samples using a silica membrane column workflow. It maintains consistent binding chemistry while reducing processing scale for moderate input volumes.

This configuration bridges micro-volume processing and larger vacuum-based workflows, offering flexibility for laboratories standardizing 1mL plasma inputs.

This BioString^TM product is based on silica gel purification. The sample is lysed and digested with lysate and protease, DNA is released into the lysate. Transfer to an adsorption plate and filter column. Nucleic acid is adsorbed on the membrane, while protein is not adsorbed and is removed with filtration. After washing proteins and other impurities, nucleic acid was finally eluted with low-salt buffer (10 Mm Tris,pH 8.0).

• High yield - most optimal process, free DNA (>50bp) can be obtained to the maximum extent
• High concentration - low elution volume, ensuring high nucleic acid concentration
• High purity - low alcohol binding method, completely removing inhibitor and protein pollution
• High recovery - DNA can be recovered at the level of PG by silica gel column purification

Proteinase K and carrier RNA should be stored at 2–8°C upon arrival. However, short-term storage (up to 12 weeks) at room temperature (15–25°C) does not affect their performance. The remaining kit components can be stored dry at room temperature (15–25°C) and are stable for at least 18 months under these conditions. The entire kit can be stored at 2–8°C, but in this case buffers should be redissolved before use. Make sure that all buffers are at room temperature when used.