Free-circulating nucleic acids, such as tumor-specific extracellular nucleic acid fragments and mRNAs in the blood or fetal nucleic acids in maternal blood, are present in serum or plasma usually as short fragments <1000bp(Nucleic Acid). The BioString^TM Circulating Nucleic acid Micro Kit enables efficient purification of these circulating nucleic acids from human plasma, serum, or urine. Samples can be either fresh or frozen (provided that they have not been frozen and thawed more than once). Free-circulating cell-free DNA, RNA or viral nucleic acids are eluted in Nuclease Free Water, ready for use in amplification reactions or storage at -30 to -15°C. Purified nucleic acids are free of proteins, nucleases, and other impurities.

This Kit is designed for purification of circulating DNA from small plasma volumes (up to 0.6mL). It applies the same silica membrane chemistry used in the Magen column-based circulating DNA system, adapted for low-input workflows.

This format is suitable for limited sample availability or preliminary testing workflows where plasma volume is restricted. The membrane configuration maintains stable adsorption performance within reduced input ranges.

This product is based onsilica column purification. The sample is lysed and digested with lysate andprotease, DNA is released into the lysate. Transfer to an adsorption plate andfilter column. Nucleic acid is adsorbed on the membrane, while protein is notadsorbed and is removed with filtration. After washing proteins and otherimpurities, Nucleic acid was finally eluted with low-salt buffer.

• High yield - most optimal process, free DNA (>50bp) can be obtained to the maximum extent
• High concentration - low elution volume, ensuring high nucleic acid concentration
• High purity - low alcohol binding method, completely removing inhibitor and protein pollution
• High recovery - DNA can be recoveredat the level of PG by silica gel column purification

Proteinase K, Carrier RNA should be stored at 2-8°C upon arrival. However, short-term storage (up to 12weeks) at room temperature (15-25°C) does not affect their performance. The remaining kit components can be stored dry at room temperature (15-25°C) and are stable for at least 18 months under these conditions. The entire kit can bestored at 2-8°C, but in this case buffers should be redissolved before use. Make sure that all buffers are at room temperature when used.