This column is configured for circulating DNA workflows where sample volume is larger and nucleic acid concentration is low. It is typically used in plasma or serum processing where recovery efficiency across milliliter-scale inputs becomes critical.

The column can be used with extended loading configurations, allowing gradual binding of fragmented DNA without compromising membrane stability.

Based on the negatively charged DNA skeleton, it has a high affinity for positively charged glass fibers. In high salt and ethanol solutions, DNA/RNA binds to glass fiber and interacts with hydrophilic matrix on silica through hydrogen bond. DNA/RNA is tightly bound. All pollutants can be removed by washing solution. At high salt concentration, nucleic acids selectively bind to silica gel membrane, while other pollutants, mainly proteins, are removed by membrane