BioString™ 2200 Transfection Reagent comprises of unique formulations of polycations and liposomes representing a new class of transfection reagent designed for primary
mammalian cells to get outstanding transfection results, which will guarantee higher transfection efficiency and lower cytotoxicity for almost all primary cell lines. BioString™ 2200 Transfection Reagent offers significant advantages over many other transfection methods.

Designed with the highest transfection efficiency, ease of use, low cytotoxicity and biodegradability, BioString™ new formulation overcomes the cytotoxic effects of common cation or lipoplasmic transfection reagents and is more suitable for long-acting and instantaneous transfection. The use of this new transfection reagent is convenient to use transfection plasmids, linear double-stranded DNA, antisense oligonucleotides and RNAi, etc., and has achieved very ideal results in actual use.

BioString™ 2200 is a transfection reagent designed for primary cell cells and can be used for in vitro transfection plasmids, linear double-stranded DNA, antisense oligonucleotides and RNAi. The transfection efficiency of commonly used primary cells can reach more than 60%.

BioString™ 2200 can form tiny (mean size about 100-400nm) single-layer liposomes, electrostatically bound to the phosphorus skeleton of DNA and the surface of negatively charged cell membranes, and can form stable small nanocolloid particles with oligonucleotides, etc., enter the cell through cell “internalization”, absorb the H+ of the lysosome, form an acidic environment in the complex to deactivate nucleases and protect DNA from nuclease. After entering the cell, the complex cysts rupture, releasing DNA into the cytoplasm to achieve gene transfection.

BioString™ 2100 Transfection Reagent offers:

• High transfection efficiency of plasmids, antisense oligonucleotides, siRNA in almost all primary cell types
• Fast procedure — transfection-complex formation in just 20 minutes
• Decreased cytotoxicity

Required amount of BioString™ 2200 Transfection Reagent

For initial optimization experiments, transfect a monolayer of cells that is 70–90% confluent, using 2:1~ 5:1 ratios of BioString™ 2200 Transfection Reagent (μl) to DNA (μg), respectively. For most cell types, the BioString™ 2200 Transfection Reagent: DNA (2:1) ratio provides excellent transfection level. For some hard-to-transfect cells, the BioString™ 2200 Transfection Reagent: DNA (5:1) ratio is recommended.

Storage and Stability

BioString™ 2200 Transfection Reagent is provided in 1 μg/μL concentration. It is shipped at room temperature and is stabilized for extended storage at +4°C for one year when tightly tightly closed.