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BCA Protein Assay Kit - Details |
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Description |
Detection and Quantification of Protein Concentration. |
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Detection Method |
Colorimetric |
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Components |
Reagent A: 25 ml x 4 Reagent B: 2 ml x 1 Standard: 2 mg x 5 |
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Storage/Stability |
Shipped and store at 4°C for 1 year. |
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Sample Types |
All |
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Platform |
Microplate reader 562 nm |
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Application Key |
H - Human, M - Mouse, R - Rat, B - Bovine, C - Chicken, D - Dog, G - Goat, Mk - Monkey, P - Pig, Rb - Rabbit, S - Sheep, Z - Zebrafish |
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Application |
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Protein quantification is often required before precessing protein samples for analysis. The Bicinchoninic Acid (BCA) Protein Assay is highly sensitive colorimetric assay that is not affected by chemicals in the sample. The BCA Protein Assay primarily reduces Cu2+ to Cu1+ by proteins in an alkaline environment followed by highly sensitive and selective colorimetric detection of BCA/copper complex. It is water-soluble and strongly absorbs light at 562nm in a linear fashion with increasing protein concentration |
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