|
ATRX (2Q5) Rabbit Monoclonal Antibody |
|
Gene Name |
ATRX |
|
Formulation |
PBS (pH 7.3) containing 1% BSA, 50% glycerol and 0.02% New type preservative N. |
|
Form of Antibody |
Liquid |
|
Isotype |
IgG |
|
Purification |
Purified from cell culture supernatant by affinity chromatography |
|
Uniprot ID |
P46100 |
|
Gene ID |
NM_000489 |
|
Clonality |
Monoclonal |
|
Immunogen |
Human recombinant protein fragment corresponding to the N-terminus of human ATRX (NP_000480) produced in E.coli. |
|
Recommended Dilutions |
IHC 1:200 |
|
Predicted Molecular Weight |
282.4kDa |
|
Storage/Stability |
Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles. |
|
Background |
The protein encoded by this gene contains an ATPase/helicase domain, and thus it belongs to the SWI/SNF family of chromatin remodeling proteins. This protein is found to undergo cell cycle-dependent phosphorylation, which regulates its nuclear matrix and chromatin association, and suggests its involvement in the gene regulation at interphase and chromosomal segregation in mitosis. Mutations in this gene are associated with X-linked syndromes exhibiting cognitive disabilities as well as alpha-thalassemia (ATRX) syndrome. These mutations have been shown to cause diverse changes in the pattern of DNA methylation, which may provide a link between chromatin remodeling, DNA methylation, and gene expression in developmental processes. Multiple alternatively spliced transcript variants encoding distinct isoforms have been reported. |
|
Validated Image |
IHC staining of FFPE human breast cancer tissue using anti-ATRX rabbit monoclonal antibody (RM1368) and Polink-2 HRP polymer detection kit . Heat-induced epitope retrieval by EDTA solution buffer pH 8.0 at 120°C for 3 min. The brown stain indicates positive stain, blue is the counter stain.
|
