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ATP Colorimetric Microplate Assay Kit |
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Applications |
Functional Assay |
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Description |
Detection and Quantification of ATP Content. |
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Detection Method |
Colorimetric |
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Sample Types |
Urine, Serum, Plasma, Tissue extracts, Cell lysate, Cell culture media, Other biological fluids |
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Platform |
Microplate reader 660 nm |
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Storage/Stability |
Shipped and store at 4°C for 6 months. |
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Alternative Names |
5'-ATP; 5'-trifosfato de adenosina; 5'-Triphosphate d'adenosine; Adenosin-5'-triphosphat; Adenosine 5'-(tetrahydrogen triphosphate); Adenosine 5'-(tetrahydrogen triphosphate); adenosine 5'-triphosphate; Adenosine 5'-triphosphoric acid; Adenosine triphosphate; Adenosine, 5'-(tetrahydrogen triphosphate); Adenylpyrophosphoric acid; Adephos; Adetol; Adynol; Atipi; Atriphos; Cardenosine; Fosfobion; Glucobasin; Myotriphos; Phosphobion; Striadyne; Triadenyl; Triphosphaden; Triphosphoric acid adenosine ester |
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Principle |
5-bromo-2-deoxyuridine (BrdU) is a pyrimidine analog. It gets incorporated into the newly synthesized DNA of proliferating cells in place of thymidine. BrdU Cell Proliferation Assay Kit detects incorporated BrdU using a rabbit anti-BrdU antibody. A Goat Anti-Rabbit IgG (H&L) - HRP secondary antibody is used to detect the anti-BrdU antibody bound to BrdU, which is followed by addition of TMB. The extent of color development is proportional to the quantity of BrdU incorporated into the cells and can be used directly as an indicator of cell proliferation. Compared to other cell proliferation assays, this kit detects only the proliferating cells and not the seeded cells |
