Directions

Dissolve 14.50 grams in 900 ml distilled water, boil to dissolve the medium completely allow to cool at room temperature and add 25ml of ethanol and dilute up to 1000 ml with distilled water. Distribute as desired in screw-capped bottles. Do not used polypropylene caps with seal. Close the caps so that the seal in the lid just touches the lip of the bottle. Sterilize by autoclaving at 15 lbs pressure (121 °C) for 15 min, cool it to 42-45 °C and inoculate test sample aseptically. Alternatively, sterilize the ethanol by filtration through the cellulose acetate or nitrate membrane of 0.22 µm and then aseptically to the medium after autoclaving and cooling.

Application

For the cultivation of Pseudomonas aeruginosa by membrane filter technique.