Description
| Product Name | anti- 15 Lipoxygenase 2 antibody |
| Catalogue No. | 50000AB |
| Size | 100µg |
| Form | liquid |
| Purification | Immunogen affinity purified |
| Purity | ≥95% as determined by SDS-PAGE |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Storage | PBS with 0.02% sodium azide and 50% glycerol pH 7.3,-20℃ for 12 months(Avoid repeated freeze / thaw cycles.) |
BACKGROUND
| In humans, there are 6 functional lipoxygenases genes. Two of them encode for arachidonic acid 12-lipoxygenating (ALOX12, ALOX12B) and two for 15-lipoxygenating enzymes (ALOX15, ALOX15B) . ALOX15B has been described as the most abundant 15-lipoxygenating enzyme species in human carotid atherosclerotic lesions and to be associated with cerebrovascular symptoms. (PMID:24373925) . ALOX15B has 4 isoforms produced by alternative splicing with the MW of 76, 67, 69 and 73 kDa. |
IMMUNOGEN INFORMATION
| Immunogen | arachidonate 15-lipoxygenase, type B |
| Observed MW | 76 kDa |
APPLICATION
| Tested Application | ELISA, WB, IHC, IF |
| Recommended Dilution | WB: 1:200-1:2000; IHC: 1:20-1:200 |
IMAGES
| Immunohistochemistry of paraffin-embedded human prostate cancer using (ALOX15B antibody) at dilution of 1:100 |  |
| MCF7 cells were subjected to SDS PAGE followed by western blot with (ALOX15B antibody) at dilution of 1:600 |  |
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