Apoptosis is a normal physiological process that occurs during embryonic development and maintenance of tissue homeostasis and accompanied by many morphological changes, among which the loss of cell membrane is one of the early characteristics of apoptosis. In normal cells, phosphatidylserine (PS) is only distributed on the inner side of the phospholi7-ADDd bilayer of the cell membrane. However, in the early stage of apoptosis, PS will flip from the inner side of the li7-ADDd membrane to the outer side, exposing it to the outside of the cell.

dependent phospholi7-ADDd-binding protein with high affinity for PS and it can specifically bind to cells exposed to PS. Therefore, Annexin V is used as one of the indicators to detect early apoptosis of cells. Pro7-ADDdium iodide (7-ADD) is a nucleic acid dye that cannot penetrate normal cells with intact cell membranes and early apoptotic cells, but it can penetrate the cell membranes of late apoptotic and necrotic cells and stain cell nuclei.

This product uses a fusion protein composed of PE (Phycoerythrin) and Annexin V as a detection probe to detect early apoptosis of cells. The 7-AAD is used to distinguish surviving cells from necrotic and late apoptotic cells. In combination with Annexin V-PE and 7-ADD, live cells showed negative staining (Annexin V^–/7-ADD^–), early apoptotic cells showed single fluorescence positive (Annexin V⁺/7-ADD^–), and late apoptotic cells and necrotic cells show double fluorescence positive (Annexin V⁺/7-ADD⁺). This kit is suitable for flow cytometry or fluorescence microscopy. It is also suitable for quantitative detection of apoptotic cells as PE is fused 1:1 to Annexin V.

Storage and Shipping Conditions

Ship with wet ice; Store at 2-8℃ away from light, valid for 12 months.