Amicon® Ultra Centrifugal Filter, 100 kDa MWCO

Catalouge No UFC910024
Synonym(s) 100 kDa MWCO, 15 mL sample volume, Centrifugal concentrator
Volume 15 mL
Sterility Non-Sterile
Pore Size 100 kDa MWCO
Manufacturer Sigma-Aldrich® Solutions
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SKU: UFC910024 Categories: ,

Amicon® Ultra 15 mL centrifugal devices with Ultracel® 100,000 Dalton molecular weight cutoff filters provide fast ultrafiltration, and reliable retention of macromolecules. For example, IgG (156 kDa) at 1 mg/ml initial concentration is retained at >90% .

The vertical design and available membrane surface area provide fast sample processing, high sample recovery (typically greater than 90% of dilute starting solution), and the capability for 80-fold concentration. Solute polarization and subsequent fouling of the membrane are minimized by the vertical design, and a physical deadstop in the filter device prevents spinning to dryness and potential sample loss.

The concentrate is collected from the filter device sample reservoir using a pipetter, while the ultrafiltrate is collected in the provided centrifuge tube. The device can be spun in a swinging bucket or fixed angle rotor. With their vertical membrane design and deadstop, Amicon® Ultra 15 mL filters deliver the highest recovery with short spin times.

We are committed to bringing you Greener Alternative Products, which have been re-engineered to enable more sustainable research solutions. The inner filtration device is made from 60% bio-circular material allocated using the ISCC mass balance approach.

Quality Level 400
product line Amicon®
packaging pkg of 24 pkg, pkg of 8 pkg, pkg of 96 pkg
sustainability Greener Alternative Product
parameter 15 mL sample volume
technique(s) protein extraction: suitable, protein purification: suitable
L 12.1 cm (4.807 in.)
diam. 2.97 cm
filtration area 7.6 cm2
greener alternative category Re-engineered
shipped in ambient

Features and Benefits

  • Ultracel® regenerated cellulose membrane with 100 kDa NMWCO delivers retentate recovery of greater than 90%
  • Vertical membrane reduces concentration polarization for ultra-fast spin times (as fast as 10–15 minutes).
  • Heat-sealed membrane minimizes downstream extractables.
  • 100% integrity tested for reliable performance.
  • Convenient sample monitoring with translucent housing and volume gradations.
  • Direct pipettor sample access eliminates processing step to recover concentrate.
  • High concentration factors of 80–100X.
  • Ultrafiltration with 100,000 Dalton molecular weight cutoff Ultracel® membrane.
  • Concentration of biological samples containing antigens, antibodies, enzymes, nucleic acids, or microorganisms.
  • Purification of macromolecular components found in tissue culture extracts or cell lysates and protein removal prior to HPLS.
  • Concentration of dilute or pre-purified proteins from column eluents.
  • Desalting, buffer exchange and protein dialysis.

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