Key Features
- Filtered through a 0.2 µm filter prior to lyophilization
- Blocking agent in Western blots or ELISA applications
Key Applications
Albumin | Biochemicals and Reagents | Bovine Serum Albumins | Ethanol Fractionated | Proteins and Derivatives
| Albumin Bovine, fraction V, ≥98% - Specifications | |
| Synonyms | BSA, Bovine albumin |
| Format | Powder / Flakes |
| Format | Powder / Flakes |
| Isoelectric Point | pI in Water at 25°C: Endogenous material - 4.7, 4.9; Fatty acid depleted- 5.3 |
| Personal Protective Equipment | Eye shields, Gloves, Respirator filter |
| Biochemical Physiological Actions | Certain conformational and primary-sequence epitopes of BSA are suspected allergens in human beef and milk allergies. |
| Albumin Bovine (pH 7) fraction V for molecular biology - Specifications | |
| Appearance (Colour) | Pale yellow to greenish yellow |
| Appearance (Form) | Crystalline compound |
| Solubility (Turbidity) 5% aq. solution | Clear |
| Solubility (Colour) 5% aq. solution | Pale yellow to greenish yellow |
| Assay (ex N) | min. 98% |
| pH (1% aq. solution) | 6.5 - 7.5 |
| Nitrogen content (on dry basis) | min. 15.4% |
| A 1% aq. | |
| @280nm | 6.34 - 6.60 |
| Water (KF) | max. 5% |
| DNase, RNase, Protease | Not detected |
Application Notes
Albumin is a general blocking reagent in ELISA, as a nutrient in cell & microbial culture, it also stabilizes some enzymes during digestion of DNA. It is also used to determine the quantity of other proteins, by comparing an unknown quantity of protein to known amounts of BSA.
Usage Statement
Unless specified otherwise, MP Biomedical’s products are for research or further manufacturing use only, not for direct human use. For more information, please contact our customer service department.
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