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Acrosin Polyclonal Antibody |
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Applications |
WB;IHC-p;IF(paraffin section);ELISA |
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Purification |
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
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Immunogen |
Synthesized peptide derived from Acrosin . at AA range: 40-120 |
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Gene Name |
ACR |
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Dilution |
IHC-p: 100-300.Western Blot: 1/500 - 1/2000. ELISA: 1/5000. Not yet tested in other applications |
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Protein Name |
Acrosin |
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Human Gene Id |
49 |
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Human Swiss Prot No |
P10323 |
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Mouse Swiss Prot No |
P23578 |
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Specificity |
Acrosin Polyclonal Antibody detects endogenous levels of Acrosin protein. |
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Formulation |
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide. |
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Concentration |
1 mg/ml |
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Alternative Name |
ACR; ACRS; Acrosin |
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Observed Band(KD) |
45kD |
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Subcellular Location |
acrosomal vesicle,extracellular region,nucleus,Golgi-associated vesicle,acrosomal matrix,protein complex, |
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Expression |
Leukocyte,Testis, |
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Function |
catalytic activity:Preferential cleavage: Arg-|-Xaa, Lys-|-Xaa.,function:Acrosin is the major protease of mammalian spermatozoa. It is a serine protease of trypsin-like cleavage specificity, it is synthesized in a zymogen form, proacrosin and stored in the acrosome.,similarity:Belongs to the peptidase S1 family.,similarity:Contains 1 peptidase S1 domain.,subunit:Heavy chain (catalytic) and a light chain linked by two disulfide bonds., |
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Background |
Acrosin is the major proteinase present in the acrosome of mature spermatozoa. It is a typical serine proteinase with trypsin-like specificity. It is stored in the acrosome in its precursor form, proacrosin. The active enzyme functions in the lysis of the zona pellucida, thus facilitating penetration of the sperm through the innermost glycoprotein layers of the ovum. The mRNA for proacrosin is synthesized only in the postmeiotic stages of spermatogenesis. In humans proacrosin first appears in the haploid spermatids. |
