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488 Anti-Mouse CD8a Antibody (53-6.7) |
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Alternative Names |
CD8A| MAL| T-cell surface glycoprotein CD8 alpha chain| T-lymphocyte differentiation antigen T8/Leu-2 antibody |
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Isotype |
IgG2a, κ |
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Form |
Liquid |
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Concentration |
0.2mg/mL |
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Conjugation |
488 |
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Clonality |
Monoclonal |
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Clone ID |
53-6.7 |
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UniProt ID |
P01731 |
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Conjugation Information |
488 is designed to be excited by the Blue laser (488 nm) and detected using an optical filter centered near 520 nm (e.g., a 525/40 nm bandpass filter). |
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Storage |
PBS with 0. 1% sodium azide, 1%BSA, pH 7.3, 2-8℃ for 12 months (Avoid repeated freeze / thaw cycles.) |
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Recommended dilution |
Each lot of this antibody is quality control tested by flow cytometric analysis. Please check your vial before the experiment. Since applications vary, the appropriate dilutions must be determined for individual use. We suggest each investigator should titrate the reagent to obtain optimal results [The recommended concentration is 0.1-1μg/10⁶ cells in 100 μL volume]. |
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Background |
CD8, also known as Lyt-2, Ly-2, or T8, consists of disulfide-linked α and β chains that form the α(CD8a)/β(CD8b) heterodimer and α/α homodimer. CD8a is a 34 kD protein that belongs to the immunoglobulin family. The CD8 α/β heterodimer is expressed on the surface of most thymocytes and a subset of mature TCR α/β T cells. CD8 expression on mature T cells is non-overlapping with CD4. The CD8 α/α homodimer is expressed on a subset of γ/δ TCR-bearing T cells, NK cells, intestinal intraepithelial lymphocytes, and lymphoid dendritic cells. CD8 is an antigen co-receptor on T cells that interacts with MHC class I on antigen-presenting cells or epithelial cells. CD8 promotes T cell activation through its association with the TCR complex and protein tyrosine kinase lck. |
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Validated Image |
C57BL/6 murine splenocytes are stained with FineTest® 488 Anti-Mouse CD8a Antibody (filled red histogram). Unstained splenocytes (empty black histogram) are used as control.
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