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488 Anti-Mouse CD25 Antibody (PC-61.5.3) |
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Alternative Names |
IL-2 receptor subunit alpha|IL-2-RA|IL-2R subunit alpha|IL2-RA|IL2RA|Interleukin-2 receptor subunit alpha|TAC antigen|p55 antibody |
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Applications |
FC |
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Clonality |
Monoclonal |
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Isotype |
IgG1, κ |
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Form |
Liquid |
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Conjugation |
488 |
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Conjugation Information |
488 is designed to be excited by the Blue laser (488 nm) and detected using an optical filter centered near 520 nm (e.g., a 525/40 nm bandpass filter). |
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Clone ID |
PC-61.5.3 |
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Storage |
PBS with 0. 1% sodium azide, 1%BSA, pH 7.3, 2-8℃ for 12 months (Avoid repeated freeze / thaw cycles.) |
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UniProt ID |
P01590 |
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Recommended dilution |
Volume per test: 5μL. Each lot of this antibody is quality control tested by flow cytometric analysis. The amount of the reagent is suggested to be used 5 µL of antibody per test (million cells in 100 µL staining volume or per 100 µL of whole blood). Please check your vial before the experiment. Since applications vary, the appropriate dilutions must be determined for individual use. |
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Background |
CD25 is a 55 kD glycoprotein, also known as the low affinity IL-2Rα, Ly-43, p55, or Tac. It is expressed on activated T and B cells, thymocyte subset, pre-B cells, and T regulatory cells. In association with CD122 (IL-2Rβ) and CD132(common γ chain), CD25 forms the high affinity signaling IL-2 receptor. |
