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10X Phosphate Buffered Saline (PBS)

10X Phosphate Buffered Saline (PBS)

Catalouge No BSCHM-062P
Size 100 ML | 500 ML
Price POR
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SKU: BSCHM-062P Categories: ,
10X Phosphate Buffered Saline (PBS), Molecular Biology
Grade Molecular Biology
Appearance (Clarity) Clear
Appearance (Colour) Colourless
Appearance (Form) Liquid
pH (10X Buffer) 6.9 ± 0.1
pH (1X Buffer) 7.4 ± 0.1
Sterility (Bacterial count) Nil
DNase, RNase, Protease Not detected

Phosphate Buffered Saline (PBS) is an essential buffer solution widely utilized in biological research and clinical settings. It plays a critical role in preventing cell rupture or shrinkage caused by osmosis, and its non-toxic formulation is safe for cellular applications. The 10X PBS solution provides a stable pH environment, which is vital for optimal enzyme activity and various biological processes. This stability makes PBS particularly suitable for preparing reagents, conducting protein dialysis, and performing immunohistochemistry, thus ensuring consistent and reliable experimental results. In addition to its applications in cell biology, PBS is also employed in diverse fields, including the development of custom ELISA buffers to optimize antibody-antigen interactions.

PBS buffer is a highly versatile solution commonly employed in a range of laboratory applications. As a phosphate-buffered saline, it is designed to maintain stable pH and osmolarity, which is crucial for protein and nucleic acid research. By preventing fluctuations in pH and osmolarity, PBS helps safeguard cell health and functionality. Its compatibility with various experimental techniques further enhances its utility, it can be used for washing and diluting cells, tissues, and proteins, as well as serving as a gentle elution buffer in many purification processes. Major applications of PBS include

  • life science and biopharma
  • Sample preparation
  • diagnostic assay manufacturing