| Goat anti-Rabbit IgG (H+L) Secondary Antibody, FITC | |
| Isotype | IgG |
| Class | Polyclonal |
| Type | Secondary Antibody |
| Conjugate | FITC |
| Excitation/Emission Max | 498/517 nm |
| Form | Lyophilized |
| Concentration | 1.5 mg/mL |
| Amount | 2 mg |
| Purification | Antigen affinity chromatography |
| Storage buffer | PBS, pH 7.6, with 15mg/mL BSA |
| Contains | 0.05% sodium azide |
| Storage conditions | 4°C, store in dark |
| Antibody Form | Whole Antibody |
| Target | IgG |
| Applications | Tested Dilution |
| Immunohistochemistry (IHC) | 1:50 - 1:200 |
| Immunocytochemistry (ICC/IF) | 1.5 µg/mL |
| Flow Cytometry (Flow) | 1:50 - 1:200 |
| Immunoprecipitation (IP) | 1:50 - 1:200 |
Goat anti-Rabbit IgG (H+L) Secondary Antibody, FITC
| Catalogue No | BSAB-027S |
| Host | Goat |
| Reactivity | Rabbit |
| Applications | IHC, ICC/IF, IP, FC |
| Size | 2 MG |
| Price | POR |
Concentration may vary slightly from lot-to-lot, see lot-specific datasheet for exact concentration. Reacts with the heavy chains of rabbit IgG and with the light chains common to most rabbit immunoglobulins, but does not react against non-immunoglobulin serum proteins. However, this antibody may cross-react with immunoglobulins from other species.
Store product protected from light at 4°C until opened. To extend the shelf-life of this product, add an equal volume of glycerol to make a final concentration of approximately 50% glycerol and store at -20°C. Fluorescein Amax= 492 nm; Emax= 520 nm. Fluorophore/Protein: 9.3 µg/mg; > 3 moles FITC per mole IgG (lot-dependent).
Reconstitute with 1.5 mL of distilled water (1.5 mg/mL after restoration).
Anti-Rabbit secondary antibodies are affinity-purified antibodies with well-characterized specificity for rabbit immunoglobulins and are useful in the detection, sorting or purification of its specified target. Secondary antibodies offer increased versatility enabling users to use many detection systems (e.g. HRP, AP, fluorescence). They can also provide greater sensitivity through signal amplification as multiple secondary antibodies can bind to a single primary antibody. Most commonly, secondary antibodies are generated by immunizing the host animal with a pooled population of immunoglobulins from the target species and can be further purified and modified (i.e. immunoaffinity chromatography, antibody fragmentation, label conjugation, etc.) to generate highly specific reagents.

Immunofluorescence analysis of Goat anti-Rabbit IgG (H+L) Secondary Antibody, FITC was performed using HeLa cells stained with alpha Tubulin Rabbit Polyclonal Primary Antibody


