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Goat anti-Rabbit IgG (H+L) Secondary Antibody, FITC

Goat anti-Rabbit IgG (H+L) Secondary Antibody, FITC

Catalogue No BSAB-027S
Host Goat
Reactivity Rabbit
Applications IHC, ICC/IF, IP, FC
Size 2 MG
Price POR
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SKU: BSAB-027S Categories: ,
Goat anti-Rabbit IgG (H+L) Secondary Antibody, FITC
Isotype IgG
Class Polyclonal
Type Secondary Antibody
Conjugate FITC
Excitation/Emission Max 498/517 nm
Form Lyophilized
Concentration 1.5 mg/mL
Amount 2 mg
Purification Antigen affinity chromatography
Storage buffer PBS, pH 7.6, with 15mg/mL BSA
Contains 0.05% sodium azide
Storage conditions 4°C, store in dark
Antibody Form Whole Antibody
Target IgG
Applications Tested Dilution
Immunohistochemistry (IHC) 1:50 - 1:200
Immunocytochemistry (ICC/IF) 1.5 µg/mL
Flow Cytometry (Flow) 1:50 - 1:200
Immunoprecipitation (IP) 1:50 - 1:200

Concentration may vary slightly from lot-to-lot, see lot-specific datasheet for exact concentration. Reacts with the heavy chains of rabbit IgG and with the light chains common to most rabbit immunoglobulins, but does not react against non-immunoglobulin serum proteins. However, this antibody may cross-react with immunoglobulins from other species.

Store product protected from light at 4°C until opened. To extend the shelf-life of this product, add an equal volume of glycerol to make a final concentration of approximately 50% glycerol and store at -20°C. Fluorescein Amax= 492 nm; Emax= 520 nm. Fluorophore/Protein: 9.3 µg/mg; > 3 moles FITC per mole IgG (lot-dependent).

Reconstitute with 1.5 mL of distilled water (1.5 mg/mL after restoration).

Anti-Rabbit secondary antibodies are affinity-purified antibodies with well-characterized specificity for rabbit immunoglobulins and are useful in the detection, sorting or purification of its specified target. Secondary antibodies offer increased versatility enabling users to use many detection systems (e.g. HRP, AP, fluorescence). They can also provide greater sensitivity through signal amplification as multiple secondary antibodies can bind to a single primary antibody. Most commonly, secondary antibodies are generated by immunizing the host animal with a pooled population of immunoglobulins from the target species and can be further purified and modified (i.e. immunoaffinity chromatography, antibody fragmentation, label conjugation, etc.) to generate highly specific reagents.


Immunofluorescence analysis of Goat anti-Rabbit IgG (H+L) Secondary Antibody, FITC was performed using HeLa cells stained with alpha Tubulin Rabbit Polyclonal Primary Antibody