| Triton X-100 | |
| Synonyms | octyl phenol ethoxylate, polyethylene glycol p- (1,1,3,3-tetramethylbutyl)-phenyl ether, polyoxyethylene octyl phenyl ether, 4-octylphenol polyethoxylate, TX-100 |
| Appearance | Colorless to light yellow slightly hazy liquid. |
| Solubility | Clear colorless solution at 5ml in 100ml of water. |
| pH (5% in water at 20°C) | 6.00-8.00 |
| FTIR (Liquid film) | Matches with the standard pattern |
| Density (at 25°C) | ~1.06 g/mL |
| Refractive index (n 20/D) | 1.490 - 1.492 |
| Water (K.F.) | <= 1.00% |
| Hydroxyl value | 85-101 |
| Residue on ignition | <= 0.40% |
| Cell Culture Test | Passes |
| Pack Size | 100 ML | 500 ML |
Triton X-100
CAS No: 9002-93-1
| Catalogue No | BSCHM-041T |
| Mol. Formula | C₃₄H₆₂O₁₁ |
| Mol. Weight | 647 |
| Price | POR |
Triton X 100 is a non-ionic, non-denaturing mild detergent with a bulky non-polar head group.
In biochemical applications, Triton X 100 is used to control protein crystallization, to prevent non-specific binding in affinity purification and in immunoassay procedures and as an additive in electrophoresis buffers.
In cell culture, Triton X 100 is used for multiple applications such as lysis of cells and intracellular organelles, solubilization of membrane proteins and lipids, and membrane permeabilization during transfection procedures. It is used typically at a concentration of 0.1% to 0.5% for lysing cells.
Although Triton X 100 is considered as a mild detergent, it can be cytotoxic to cells if used in excess amount or, if cells are exposed to it for prolonged duration. Toxicity of Triton X-100 is attributed to disrupting action of its polar head group on hydrogen bonding present within the cell's lipid bilayer.