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8-OHdG (8-Hydroxydeoxyguanosine) ELISA Kit |
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Sensitivity |
0.94 ng/mL |
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Range |
1.56-100 ng/mL |
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Alternative Names |
7,8-Dihydro-8-oxo-2'-deoxyguanosine; 7,8-Dihydro-8-oxodeoxyguanosine; 8-Hydroxy-2'-deoxyguanosine; 8-Hydroxydeoxyguanosine; 8-Oxo-2'-deoxyguanosine |
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Detection Method |
Competitive |
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Standard |
100 ng/mL |
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Sample Type |
Serum, Plasma, Tissue homogenates, Cell lysates, Cell culture supernates and Other biological fluids, Sample volume: 50μL |
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Reaction time |
1.5H |
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Research Area |
Metabolism, Cardiovascular, Immunology, Cancer |
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Principle |
This ELISA kit uses the Competitive-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with 8-OHdG. Standards or samples are added to the appropriate micro ELISA plate wells then with a biotin-conjugated antibody specific to 8-OHdG. Next,Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of 8-OHdG in the samples is then determined by comparing the OD of the samples to the standard curve. |
