One Step RT-PCR Kit is designed for simplicity and convenience in carrying out RT-PCR in a one-tube format. Using this convenient one-step formulation, performing both cDNA synthesis and PCR amplification in a single tube using gene-specific primers and target RNAs has become very easy. The One-Step RT-PCR Kit comes complete and ready to use for RT-PCR. The kit can be used with diverse RNA samples and custom primers. The kit uses M-MuLV Reverse Transcriptase and Taq DNA Polymerase, premixed together at concentrations optimized to balance sensitivity and specificity. One Step RT-PCR Kit (Semi-Q PCR Based) combines reverse transcription and RNA amplification in a single tube reaction. This setup facilitates molecular virology screening and transcript detection using semi quantitative gel based analysis.

Owing to its good amplification efficiency, specificity and stability, it can greatly improve the sensitivity. The product is designed for convenient, good sensitivity one step RT‐PCR reaction. Its unique enzyme and tailor‐made buffer can ensure the efficiency and accuracy of reverse transcription PCR reaction.