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Mouse Granulocyte-Macrophage Colony Stimulating Factor(GM-CSF) ELISA Kit |
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Sensitivity |
5.2 pg/mL |
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Range |
15.6-1000 pg/mL |
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Alternative Names |
CSF2; myeloid progenitor growth factor |
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Detection Method |
Quantitative Determination |
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Storage |
-20℃(Short-term should be stored at 4℃,such as 2 weeks). |
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Sample Type |
Serum, Plasma, Tissue homogenates, Cell lysates, Cell culture supernates and Other biological fluids |
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Principle |
Detection Principle of Granulocyte-Macrophage Colony Stimulating Factor(GM-CSF) ELISA Kit As mentioned above, this kit utilizes the Double Antibody Sandwich ELISA technique. The pre-coated antibody is an anti-Granulocyte-Macrophage Colony Stimulating Factor(GM-CSF) monoclonal antibody, while the detection antibody is a biotinylated polyclonal antibody. Samples and biotinylated antibodies are added to the ELISA plate wells, and each addition is followed by washing with PBS or TBS. Then avidin-peroxidase conjugates are added to the wells. After thoroughly washing away the unbound enzyme conjugates with PBS or TBS, TMB substrate is added for color development .TMB is catalyzed by peroxidase to form a blue product, which finally turns yellow after the addition of stop solution (Color Reagent C). The color intensity is positively correlated with the concentration of the target analyte in the sample. |
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Mouse Granulocyte-Macrophage Colony Stimulating Factor(GM-CSF) ELISA Kit |
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Sensitivity |
9.375pg/ml |
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Range |
15.625-1000pg/ml (1pg=350mIU) |
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Alternative Names |
Granulocyte-macrophage colony-stimulating factor ELISA Kit, GM-CSF ELISA Kit, Colony-stimulating factor ELISA Kit, CSF ELISA Kit, CSF2 ELISA Kit, Csfgm ELISA Kit |
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UniProt ID |
P01587 |
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Sample Type |
Serum, Plasma, Cell Culture Supernatant, cell or tissue lysate, Other liquid samples |
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Detection Method |
Sandwich ELISA, Double Antibody |
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Detection Wavelength |
OD450 |
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Reaction Duration |
4 hours |
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Specificity |
Specifically binds with GM-CSF , no obvious cross reaction with other analogues. |
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Storage |
2-8°C(Sealed), Don't cryopreserve. |
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Mouse Granulocyte-Macrophage Colony Stimulating Factor(GM-CSF) ELISA Kit |
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Sensitivity |
0.04 pg/mL |
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Range |
0.78-50pg/mL |
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Alternative Names |
GMCSF, CSF2 |
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UniProt ID |
P01587 |
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Sample Type |
Serum, Plasma, Tissue homogenate and Other biological samples；Sample Volume=100μL |
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Detection Method |
Sandwich |
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Standard |
50pg/mL |
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Reaction Duration |
3.5H |
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Research Area |
Metabolic pathway;Infection immunity;Nutrition metabolism; |
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Principle |
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Mouse GM-CSF. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Mouse GM-CSF and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Mouse GM-CSF, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Mouse GM-CSF. You can calculate the concentration of Mouse GM-CSF in the samples by comparing the OD of the samples to the standard curve. |
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Mouse Granulocyte-Macrophage Colony Stimulating Factor(GM-CSF) ELISA Kit |
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Range |
31.2 pg/ml - 2000 pg/ml |
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Alternative Names |
GMCSF; Granulocyte-macrophage colony-stimulating factor; GM-CSF; Colony-stimulating factor; CSF; Molgramostin; Sargramostim |
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Description |
Sandwich ELISA for quantitative detection of Mouse GM-CSF concentrations. |
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Sample Type |
Cell Culture Supernates, Serum, Plasma |
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Gene Symbol |
CSF2 |
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Entrez Gene (Mouse) |
12981 |
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SwissProt (Mouse) |
P01587 |
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Detection Method |
Colorimetric |
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Storage/Stability |
Shipped and store at 4°C for 6 months, store at -20°C for one year. Avoid freeze/thaw cycles. |
