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Goat anti-Rabbit IgG (H+L) Secondary Antibody, HRP

Goat anti-Rabbit IgG (H+L) Secondary Antibody, HRP

Catalogue No BSAB-025S
Host Goat
Reactivity Rabbit
Applications WB, IHC (P)
Size 2 ML
Price POR
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SKU: BSAB-025S Categories: ,
Goat anti-Rabbit IgG (H+L) Secondary Antibody, HRP
Isotype IgG
Class Polyclonal
Type Secondary Antibody
Immunogen Purified Rabbit IgG, whole molecule
Conjugate HRP
Form Lyophilized
Concentration 0.8 mg/mL
Purification Antigen affinity chromatography
Storage buffer PBS, pH 7.6, with 15mg/mL BSA, 50mM sucrose
Contains no preservative
Storage conditions 4°C
Target IgG
Antibody Form Whole Antibody
Applications Tested Dilution
Western Blot (WB) 1:10,000-1:200,000
Immunohistochemistry (Paraffin) (IHC (P)) Assay-dependent
ELISA (ELISA) 1:5,000-1:10,000
Immunoprecipitation (IP) 1:500-1:5,000

This product reacts with the heavy chains of rabbit IgG and the light chains common to most rabbit immunoglobulins. It does not react with non-immunoglobulin serum proteins. However, cross-reactivity may occur with immunoglobulins from other species.

Reconstitution and Storage Guidelines

Short-term storage (≤1 week): Reconstitute with 2 mL distilled water. Store at 2-8°C.
Long-term storage: Reconstitute with 1 mL distilled water, add 1 mL glycerol, and store at -20°C. Stable for up to 1 year.

Anti-Rabbit secondary antibodies are affinity-purified antibodies with well-characterized specificity for rabbit immunoglobulins and are useful in the detection, sorting or purification of its specified target. Secondary antibodies offer increased versatility enabling users to use many detection systems (e.g. HRP, AP, fluorescence). They can also provide greater sensitivity through signal amplification as multiple secondary antibodies can bind to a single primary antibody. Most commonly, secondary antibodies are generated by immunizing the host animal with a pooled population of immunoglobulins from the target species and can be further purified and modified (i.e. immunoaffinity chromatography, antibody fragmentation, label conjugation, etc.) to generate highly specific reagents.