| Goat anti-Rabbit IgG (H+L) Secondary Antibody, HRP | |
| Isotype | IgG |
| Class | Polyclonal |
| Type | Secondary Antibody |
| Immunogen | Purified Rabbit IgG, whole molecule |
| Conjugate | HRP |
| Form | Lyophilized |
| Concentration | 0.8 mg/mL |
| Purification | Antigen affinity chromatography |
| Storage buffer | PBS, pH 7.6, with 15mg/mL BSA, 50mM sucrose |
| Contains | no preservative |
| Storage conditions | 4°C |
| Target | IgG |
| Antibody Form | Whole Antibody |
| Applications | Tested Dilution |
| Western Blot (WB) | 1:10,000-1:200,000 |
| Immunohistochemistry (Paraffin) (IHC (P)) | Assay-dependent |
| ELISA (ELISA) | 1:5,000-1:10,000 |
| Immunoprecipitation (IP) | 1:500-1:5,000 |
Goat anti-Rabbit IgG (H+L) Secondary Antibody, HRP
| Catalogue No | BSAB-025S |
| Host | Goat |
| Reactivity | Rabbit |
| Applications | WB, IHC (P) |
| Size | 2 ML |
| Price | POR |
This product reacts with the heavy chains of rabbit IgG and the light chains common to most rabbit immunoglobulins. It does not react with non-immunoglobulin serum proteins. However, cross-reactivity may occur with immunoglobulins from other species.
Reconstitution and Storage Guidelines
Short-term storage (≤1 week): Reconstitute with 2 mL distilled water. Store at 2-8°C.
Long-term storage: Reconstitute with 1 mL distilled water, add 1 mL glycerol, and store at -20°C. Stable for up to 1 year.
Anti-Rabbit secondary antibodies are affinity-purified antibodies with well-characterized specificity for rabbit immunoglobulins and are useful in the detection, sorting or purification of its specified target. Secondary antibodies offer increased versatility enabling users to use many detection systems (e.g. HRP, AP, fluorescence). They can also provide greater sensitivity through signal amplification as multiple secondary antibodies can bind to a single primary antibody. Most commonly, secondary antibodies are generated by immunizing the host animal with a pooled population of immunoglobulins from the target species and can be further purified and modified (i.e. immunoaffinity chromatography, antibody fragmentation, label conjugation, etc.) to generate highly specific reagents.
Western blot analysis was performed on whole cell extracts (30 µg lysate)
Western blot analysis of Pax4 was performed by loading the indicated amounts of recombinant Pax3 (negative control) or Pax4 proteins, and 10 µL of PageRuler Prestained Protein Ladder
Western blot analysis of PCNA was performed by loading 50 µg of the indicated whole cell lysates per well, and 10 µL of PageRuler Plus Prestained Protein Ladder


