SerRed is a highly sensitive, non-mutagenic, ultra-safe and ultra-stable fluorescent nucleic acid gel staining reagent (working concentration), which can replace ethidium bromide (EtBr, EB) with much higher sensitivity than EB without decolorization. SerRed has the same spectral characteristics as EB, and replaces EB without changing the imaging system.
SerRed Nucleic Acid Dye
| Catalogue No | BSCHM-060S |
| Storage | Room Temperature |
| Pack Size | 100UL |
| Price | POR |
Store and transport at room temperature away from light; valid for 24 months.
Glue dyeing method (same usage as EB)
- To prepare the gel, add 5 µL of SerRed Nucleic Acid Dye (10,000×, water soluble) per 50 mL of agarose (G5056 recommended) gel and mix thoroughly (SerRed has excellent thermal stability and can be added directly to the high-temperature gel solution without waiting for the gel solution to cool. It can also be prepared by pre-mixing SerRed with an electrophoresis buffer containing agarose powder and heating it).
- Electrophoresis according to conventional methods.
Soak dyeing method
- Electrophoresis according to conventional methods.
- Dilute SerRed nucleic acid dye (10,000×, water soluble) 10,000 times with 1×TAE or TBE to make 1×staining solution (for example, to prepare 50 mL of dye solution, add 5 µL SerBlue nucleic acid dye (10,000×, water-soluble) to 50 mL of 1×TAE or TBE and mix well for later use).
- Carefully place the gel into a suitable container and soak the gel with a sufficient amount of 1×staining solution. In order to shorten the soaking time, the dyeing solution can be pre-heated to about 70℃, then put into the gel and incubate for 10 min to obtain the ideal effect (If not heated, incubate for 30 min in room temperature shaker; if it is acrylamide gel, incubate for 30-60 min and extend with the increase of acrylamide content).The amount of bubble dyeing dye is large, and the dyeing solution can be reused for about 3 times for a single use. The 1×SerRed stain solution can be prepared in large quantities and stored at room temperature away from light until used up.
