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Simian Anti-Herpes B Virus IgG (HBV-IgG) Elisa Kit

Simian Anti-Herpes B Virus IgG (HBV-IgG) Elisa Kit

Catalogue No BSEK-306O
Size 96 T | 48 T
Price POR
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SKU: BSEK-306O Categories: ,
Simian Anti-Herpes B Virus IgG (HBV-IgG) ELISA Kit
Qualitative Negative/Positive
Sample Volume 10ul
Detection Method Indirect ELISA
Wavelength 450nm
Sample Type In serum, plasma, culture media or any biological fluid
Storage 2-8℃.
Principle The ELISA is based on the the qualitative enzyme immunoassay technique.The Microplateprovided in this kit has been pre-coated with an antigen specific to HBV-Ab, makeit tosolid-phase antigen.Samples are added to the Microplate wells and combined to the specificantigen. Then a Horseradish Peroxidase (HRP)-conjugated antigen specific for HBV-Abisadded to each Microplate well and incubated,so the antigen-antibody-Enzyme labeledantigencomplex is formed.Following a wash to remove any unbound reagent,then the TMBsubstratesolution is added to each well. Only those wells that contain HBV-Ab and HRP conjugatedHBV antigen will appear blue in color and then turn yellow after the addition of thestopsolution. The optical density (OD) is measured spectrophotometrically at a wavelengthof 450nm. The qualitative determination of HBV-Ab is determined by comparing with the CUTOFFvalue

Our Simian Anti-Herpes B Virus Virus (HBV-Ab)ELISA Kit is to for the qualitativedetermination of HBV-Ab in Simian serum, plasma, culture media or any biological fluid.