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Rat Cyclic Adenosine Monophosphate, cAMP ELISA Kit |
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Sensitivity |
0.06ng/ml |
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Range |
0.312ng/ml-20ng/ml |
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Detection Method |
Quantitative Detection |
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Sample Type |
with serum, plasma, culture media, or any biological fluids. |
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Storage |
2-8℃ |
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Principle |
The quantitative detection of Rat cAMP antigen is based on the ELISA (Enzyme-Linked Immunosorbent Assay) technique. The microplate wells are pre-coated with an antibody specific to Rat cAMP. After incubation, the corresponding antigen in the sample or standards will bind to the immobilized antibody. After washing the microplate wells to remove all unbound sample material, horseradish peroxidase (HRP) labeled antibody conjugate is added, which binds to the antigen, forming antibody-antigen-enzyme-labeled antibody complex upon incubation. In a second wash step, all unbound material is removed. The immune complex formed by the bound conjugate is visualized by adding Tetramethylbenzidine (TMB) substrate, which gives a blue color reaction in wells containing the complex. A stop solution containing acid terminates the reaction, producing a yellow end-point coloration proportional to the amount of specific antigen present in the sample. Absorbance is measured at 450 nm using an ELISA microwell plate reader. Analysis is completed by comparing the OD values of samples to the standard curve. |
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Rat Cyclic Adenosine Monophosphate, cAMP ELISA Kit |
|
Sensitivity |
143.2pg/mL |
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Range |
312.5-20000pg/mL |
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Detection Method |
Competitive Inhibition |
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Sample Type |
with serum, plasma, culture media, or any biological fluids. |
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Storage |
2-8℃ |
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Assay Length |
2.5h |
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Principle |
This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Cyclic Adenosine Monophosphate(cAMP) protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Cyclic Adenosine Monophosphate(cAMP). Next,Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Cyclic Adenosine Monophosphate(cAMP) in the samples is then determined by comparing the OD of the samples to the standard curve. |
