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Rat Bcl2 (B-Cell Leukemia/Lymphoma 2) ELISA Kit |
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Sensitivity |
29 pg/mL |
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Range |
78.13-5000 pg/mL |
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Detection Method |
Sandwich |
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Sample Type |
serum, plasma, tissue homogenates and other biological fluids |
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Alternative Names |
B-Cell CLL/Lymphoma 2 |
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Assay length |
3.5h |
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Standard |
5000 pg/mL |
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Research Area |
Signal transduction; Apoptosis |
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Principle |
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Rat Bcl2. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat Bcl2. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Rat Bcl2, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulfuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rat Bcl2 |
