| Human Periostin (POSTN) Elisa Kit | |
| Sensitivity | 5.6pg/ml |
| Range | 16 pg/ml -1000 pg/ml |
| Detection Method | Quantitative Detection |
| Wavelength | 450nm |
| Standard | 1350 pg/m |
| Sample Type | use with serum, plasma, culture media, or any biological fluid |
| Sample Volume | 10ul |
| Principle | The quantitative detection of Human POSTN antigen is based on the ELISA (Enzyme-Linked Immunosorbent Assay) technique. The microplate wells are pre-coated with an antibody specific to Human POSTN. After incubation, the corresponding antigen in the sample or standards will bind to the immobilized antibody. After washing the microplate wells to remove all unbound sample material, horseradish peroxidase (HRP) labeled antibody conjugate is added, which binds to the antigen, forming antibody-antigen-enzyme-labeled antibody complex upon incubation. In a second wash step, all unbound material is removed. The immune complex formed by the bound conjugate is visualized by adding Tetramethylbenzidine (TMB) substrate, which gives a blue color reaction in wells containing the complex. A stop solution containing acid terminates the reaction, producing a yellow end-point coloration proportional to the amount of specific antigen present in the sample. Absorbance is measured at 450 nm using an ELISA microwell plate reader. Analysis is completed by comparing the OD values of samples to the standard curve. |
Human Periostin (POSTN) Elisa Kit
| Catalogue No | BSEK-265H |
| Species | Human |
| Size | 96 T | 48 T |
| Price | POR |
Intra-assay Precision (Precision within an assay): 3 samples with low, middle, and high levels of Human POSTN were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle, and high-level Human POSTN were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/mean X 100
Intra-Assay: CV<10%
Inter-Assay: CV<12%
Enzyme immunoassay for the quantitative detection of Human POSTN antigen in serum, plasma, culture media, or any biological fluid.