Immediate RNase inactivation

• If any precipitation of RNA Guard Solution is seen, heat it to 37°C to redissolve it.
• If still crystal were not dissolve loosen the cap and heat the solution up to 65°C for 30 minutes mix periodically.
• Make smaller aliquots.
• Heating the solution will does not affect performance of solution

• Use RNA Guard Solution with fresh tissue only; do not freeze tissues before immersion in RNA Guard Solution.
• Before immersion in the solution make sure to cut the large tissues in small
• Place the fresh tissue in 5- 15 volumes of RNA Guard
• Most of the samples in RNA Guard can be store at room temperature for up to 1 week without compromising the quality of RNA and can be kept at –20°C for longer

Do not freeze samples in RNA Guard Solution immediately;

store at 4°C overnight (to allow the solution to penetrate within the tissue), remove supernatant, then move to–20°C for long-term storage.

Animal tissue, Plant tissue, Tissue culture cells, Blood and plasma, Yeast and Bacteria can be stored easily.