Suitable for gravity columns and prepacked columns; features high flow rate, high binding capacity, and low Ni2+ leaching; can chelate various metal ions for diverse applications.
Ni-NTA SweAgrose 6 Fast Flow is a metal ion affinity chromatography separation medium formed by immobilizing NTA ligands chelated with nickel ions on agarose microspheres. Immobilized Metal Ion Affinity Chromatography (IMAC, NTA) separates and purifies proteins by exploiting the interactions between Ni²⁺and specific amino acid side chains on proteins (mainly His and to a lesser extent Cys and Trp).
| Indicator Name | Description |
| Product Content | 66% (v/v) agarose gel, stored in 20% ethanol |
| Resin type | 6% cross-linked agarose gel |
| Base pairing | -NTA- Ni2+ |
| Particle size range (μm) | 45 - 165 |
| Average particle size (μm) | 90 |
| Protein binding capacity | 20-50 mg (His tagged protein)/mL |
| Ligand density (μmol/mL) | ~ 25 |
| Operating flow rate (cm/h) | ≤600 |
| Pressure resistance (MPa) | 0.3 |
| Storage | 2-8 ℃ 20% ethanol |
| Chemical stability range | Commonly used aqueous buffer solutions; 0.01 M hydrochloric acid; 0.1 M sodium hydroxide (1 week); 1 M sodium hydroxide, 70% acetic acid (1 day); 2% SDS (1 hour); 30% 2-propanol (30 minutes) |
Compatibility of Reagents
Buffer solution: 50 mM sodium phosphate, pH 7.4 | 100 mM Tris-HCl, pH 7.4 | 100 mM Tris-acetate, pH 7.4 | 100 mM HEPES, pH 7.4 | 100 mM MOPS, pH 7.4 | 100 mM sodium acetate, pH 4
Denaturing agent: 8 M Urea | 6 M Gua-HCl
Detergent: 2% Triton X-100 | 2% Tween 20 | 2% NP-40 | 2% Cholate | 1% CHAPS
Reducing agent: 5 mM DTE | 2 mM DTT | 20 mM β-mercaptoethanol | 5 mM TCEP | 10 mM reduced glutathione
Other additives: 500 mM Imidazole | 20% Ethanol | 50% Glycerol | 100 mM Na2SO4 | 1.5 M NaCl | 1 mM EDTA | 60 mM Citrate