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488 Anti-Mouse CD4 Antibody(GK1.5)

488 Anti-Mouse CD4 Antibody(GK1.5)

Catalogue No BSPC-033FA
Host Rat
Reactivity Mouse
Size 25μg | 100μg
Price POR
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SKU: BSPC-033FA Categories: ,
488 Anti-Mouse CD4 Antibody(GK1.5)
Alternative Names L3T4| T-cell surface antigen T4/Leu-3| T-cell surface glycoprotein CD4| T4 antibody
Applications FC
Clonality Monoclonal
Isotype IgG2b, κ
Form Liquid
Concentration 0.2mg/mL
Conjugation 488
Conjugation Information 488 is designed to be excited by the Blue laser (488 nm) and detected using an optical filter centered near 520 nm (e.g., a 525/40 nm bandpass filter).
Clone ID GK1.5
Storage PBS with 0. 1% sodium azide, 1%BSA, pH 7.3, 2-8℃ for 12 months (Avoid repeated freeze / thaw cycles.)
UniProt ID P06332
Recommended dilution Each lot of this antibody is quality control tested by flow cytometric analysis. Please check your vial before the experiment. Since applications vary, the appropriate dilutions must be determined for individual use. We suggest each investigator should titrate the reagent to obtain optimal results [The recommended concentration is 0.1-1μg/10⁶ cells in 100 μL volume].
Background CD4 is a 55 kD protein also known as L3T4 or T4. It is a member of the Ig superfamily, primarily expressed on most thymocytes, a subset of T cells, and weakly on macrophages and dendritic cells. It acts as a coreceptor with the TCR during T cell activation and thymic differentiation by binding MHC class II and associating with the protein tyrosin kinase, lck.

KM mouse splenocytes are stained with 488 Anti-Mouse CD4 Antibody (red histogram). Unstained splenocytes ( black histogram) are used as control.