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TNF-α Detection ELISA Kit (High Sensitive) |
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Sensitivity |
< 0.52pg/ml |
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Range |
1.56-100pg/ml |
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Accuracy & Precision |
Intra-Assay: CV<10% Inter-Assay: CV<15% |
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Assay Matrix |
Human serum and plasma |
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Storage |
Shipped at 2-8°C. Longterm Storage at -20° C |
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Description |
The kit is a sandwich enzyme immunoassay for in vitro quantitative measurement of TNF alpha in human serum, plasma, tissue homogenates, cell lysates, cell culture supernatants and other biological fluids. |
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Principle |
The microplate provided in this kit has been pre-coated with an antibody specific to TNFa. Standards or samples are then added to the appropriate microplate wells with a biotin-conjugated antibody specific to TNFa. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain TNFa, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the
addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of TNFa in the samples is then determined by comparing the O.D. of the samples to the standard curve. |
