🚀 Multiplex Customized ELISA Kits – Accuracy, Efficiency, Innovation in Every Assay!

PCR Master Mix (Blue)

PCR Master Mix (Blue)

Catalouge No BSEQ004
Specification 1mL
Storage -20C°
Shelf life two years
Price POR
Contact Us

PCR Master Mix is a ready-to-use, conventional PCR premix solution containing Taq DNA Polymerase, dNTP mixture, MgCl2 and an optimized buffer system. The reaction can be carried out by simply adding primers and templates, which greatly simplifies the experimental procedure. The product contains bromophenol blue dye, and the PCR product can be directly electrophoresed. This product contains excellent stabilizers and can beplacedfor3months at 4 °C. The PCR product has a 3′-dA overhang and can be easily cloned into a T vector.

Reaction System
Components Volume(μL)
PCR Master Mix (Blue) 25
Primer 1(10 μM) 1
Primer 2(10 μM) 1
Template Moderate amount
ddH2O up to 50
Amplification Procedure
Cycle step Temperature(℃) Time Cyclenumber
Pre-denaturation 94 1-5 min 1x
Denaturation 94 30 sec 35
Annealing 50-60 30 sec
Extended 72 30 sec
Final Extended 72 10 min 1x

Be sure to mix thoroughly before use.

  • Template usage: genomic DNA: 50-200 ng; plasmid DNA: 0.1-10 ng.
  • Annealing temperature: Please refer to the theoretical Tm value of the primer, and the annealing temperature can be set lower than the theoretical value of the primer by 2-5°C.
  • Extension time: Molecular identification is recommended at 30 sec/kb. Gene cloning is recommended at 60 sec/kb to ensure the highest amount of product.