| Reaction System | |||
| Components | Volume（μL） | ||
| PCR Master Mix (Red) | 25 | ||
| Primer 1(10 μM) | 1 | ||
| Primer 2(10 μM) | 1 | ||
| Template | Moderate amount | ||
| ddH2O | up to 50 | ||
| Amplification Procedure | |||
| Cycle step | Temperature（℃） | Time | Cyclenumber |
| Pre-denaturation | 94 | 1-5 min | 1 |
| Denaturation | 94 | 30 sec | 35 |
| Annealing | 50-60 | 30 sec | |
| Extended | 72 | 30 sec | |
| Final Extended | 72 | 10 min | 1 |
PCR Master Mix(Red)
| Catalouge No | BSEQ027 |
| Specification | 1mL |
| Storage | -20C° |
| Shelf life | two years |
| Price | POR |
Be sure to mix it thoroughly before use. a. Template usage amount: genomicDNA:50-200 ng; plasmid DNA: 0.1-10 ng. b. Annealing temperature: Please refer to the theoretical Tm value of the primer. The annealing temperature can be set 2-5℃lowerthan the theoretical value of the primer. c. Extension time: 30 sec/kb is recommended for molecular identification. Gene cloning recommends 60 sec/kb to ensure maximum product yield.