Nile red - used for the detection of intracellular lipid droplets by fluorescence microscopy and flow cytofluorometry. Nile Red has been shown to detect the exposure or formation of new hydrophobic surfaces induced by ligand binding to calmodulin, oligomerization of melittin, or unfolding of ovalbumin during early thermal denaturation.

Nile red, for microscopy  - used to detect intracellular lipid droplets and proteins.

 Nile red, puriss- used for staining SDS (sodium dodecyl sulphate) gels.  Nile red, puriss  - used as a membrane dye which can be readily visualized using an epifluorescence microscope with excitation and emission wavelengths usually shared with red fluorescent protein.