SYBR® Green qPCR Mix is designed for high-performance, high-throughput real-time PCR. The kit contains Taq DNA Polymerase engineered through a process of molecular evolution. The result is a unique polymerase that specifically designed for qPCR using SYBR® Green I chemistry dye. 2X SYBR® Green qPCR Mix is a convenient premix of the components (except primers and DNA template) that necessary to perform real-time polymerase chain reaction (PCR) using SYBR® Green I dye with enhanced sensitivity and specificity. The SYBR® Green I dye binds to double-stranded DNA (dsDNA), thus providing a fluorescent signal that reflects the amount of dsDNA products generated during PCR.
Applications
- Gene expression analysis
- Low-copy gene detection
- Microarray validation
- Gene knockdown validation
Features
- Compatible with many Real-time systems which not require ROX reference dye
- Hot-start technology brings high specificity and reproducible amplification
Storage
This reagent can be stored for 2 months at 4°C and protected from light. For longer storage, it should be kept at -20°C and protected from light.