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PE-Cy7 Anti-Human CD279/PD-1 Antibody(EH12.2H7) - Details |
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Synonyms |
PD1|PDCD1|Protein PD-1|hPD-1 antibody |
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Clonality |
Monoclonal |
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Isotype |
IgG1, Īŗ |
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Form |
Liquid |
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Conjugation |
PE-Cy7 |
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Conjugation Information |
PE-Cy7 is designed to be excited by the Blue (488 nm), Green (532 nm) and yellow-green (561 nm) lasers and detected using an optical filter centered near 775 nm (e.g., a 780/60 nm bandpass filter). |
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Clone ID |
EH12.2H7 |
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Storage |
PBS with 0. 1% sodium azide, 1%BSA, pH 7.3, 2-8ā for 12 months (Avoid repeated freeze / thaw cycles.) |
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UniProt ID |
Q15116 |
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Recommended dilution |
Volume per test: 5Ī¼L. Each lot of this antibody is quality control tested by flow cytometric analysis. The amount of the reagent is suggested to be used 5 ĀµL of antibody per test (million cells in 100 ĀµL staining volume or per 100 ĀµL of whole blood). Please check your vial before the experiment. Since applications vary, the appropriate dilutions must be determined for individual use. |
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Background |
Programmed cell death 1 (PD-1), also known as CD279, is a 55 kD member of the immunoglobulin superfamily. CD279 contains the immunoreceptor tyrosine-based inhibitory motif (ITIM) in the cytoplasmic region and plays a key role in peripheral tolerance and autoimmune disease. CD279 is expressed predominantly on activated T cells, B cells, and myeloid cells. PD-L1 and PD-L2 are ligands of CD279 (PD-1) and are members of the B7 gene family. Evidence suggests overlapping functions for these two PD-1 ligands and their constitutive expression on some normal tissues and upregulation on activated antigen-presenting cells. Interaction of CD279 ligands results in inhibition of T cell proliferation and cytokine secretion. |
