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FITC Anti-Mouse CD45 Antibody - Details |
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Synonyms |
CD45| L-CA| Ly-5| Ptprc| Receptor-type tyrosine-protein phosphatase C| T200 antibody |
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Clonality |
Monoclonal |
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Isotype |
IgG2b, Īŗ |
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Form |
Liquid |
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Concentration |
0.2mg/mL |
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Conjugation Information |
FITC is designed to be excited by the Blue laser (488 nm) and detected using an optical filter centered near 530 nm (e.g., a 525/40 nm bandpass filter). |
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Clone ID |
30-F11 |
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Storage |
PBS with 0. 1% sodium azide, 1%BSA, pH 7.3, 2-8ā for 12 months (Avoid repeated freeze / thaw cycles.) |
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Alternative Names |
CD45| L-CA| Ly-5| Ptprc| Receptor-type tyrosine-protein phosphatase C| T200 antibody |
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UniProt ID |
P06800 |
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Recommended dilution |
Each lot of this antibody is quality control tested by flow cytometric analysis. Please check your vial before the experiment. Since applications vary, the appropriate dilutions must be determined for individual use. We suggest each investigator should titrate the reagent to obtain optimal results [The recommended concentration is 0.1-1Ī¼g/10ā¶ cells in 100 Ī¼L volume]. |
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Background |
CD45 is a 180-240 kD glycoprotein also known as the leukocyte common antigen (LCA), T200, or Ly-5. It is a member of the protein tyrosine phosphatase (PTP) family, expressed on all hematopoietic cells except mature erythrocytes and platelets. There are different isoforms of CD45 that arise from alternative splicing of exons 4, 5, and 6, which encode A, B, and C determinants, respectively. CD45 plays a key role in TCR and BCR signal transduction. These isoforms are very specific to the activation and maturation state of the cell as well as cell type. The primary ligands for CD45 are galectin-1, CD2, CD3, CD4, TCR, CD22, and Thy-1. |
