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Human IL-17A(Interleukin 17A) ELISA Kit

Catalogue No BSEK-192H
Species Human
Size 96 T | 48 T
Price POR
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SKU: BSEK-192H Categories: ,
Human IL-17A (Interleukin 17A) ELISA Kit - Details
Sensitivity 4.69pg/mL
Range 7.81-500pg/mL
Uniprot ID Q16552
Alternative Names CTLA8, CTLA-8, CTLA8cytotoxic T-lymphocyte-associated serine esterase 8, Cytotoxic T-lymphocyte-associated antigen 8, IL17, IL-17, IL17A, IL-17A, IL-17Acytotoxic T-lymphocyte-associated protein 8, IL-17CTLA-8
Detection Method Sandwich
Standard 500pg/mL
Sample type Serum, Plasma and Other biological fluids;Sample Volume=100μL
Reaction time 3.5H
Research Area Cancer,Immunology
Principle This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human IL-17A. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human IL-17A and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human IL-17A, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Human IL-17A. You can calculate the concentration of Human IL-17A in the samples by comparing the OD of the samples to the standard curve.