| Human CD8A (T-cell surface glycoprotein CD8 alpha chain) ELISA Kit - Details | |
| Sensitivity | 37.5pg/ml |
| Range | 62.5-4000pg/ml |
| Uniprot ID | P01732 |
| Alternative Names | CD8A ELISA Kit, T-cell surface glycoprotein CD8 alpha chain ELISA Kit, T-lymphocyte differentiation antigen T8 ELISA Kit, Leu-2 ELISA Kit |
| Detection Method | Sandwich ELISA, Double Antibody |
| Detection Wavelength | OD450 |
| Sample type | Serum, Plasma, Cell Culture Supernatant, cell or tissue lysate, Other liquid samples |
| Reaction time | 4 hours |
| Specificity | Specifically binds with CD8A , no obvious cross reaction with other analogues. |
| Principle | This kit is based on Double antibody-Sandwich ELISA detection method and takes 4h assay time. The microplate provided in this kit has been precoated with anti CD8A antibody. Add standard and properly diluted sample into relevant well respectively. After incubation, wash unbound components. Add biotinylated detection antibody. Then, it binds with CD8A bound to precoated antibody. Wash unbound components and add HRP-Streptavidin Conjugate (SABC). Wash unbound components again and add TMB substrate solution. Then, TMB was catalyzed by HRP to produce a blue color product that turned yellow after adding a stop solution. Read the O.D. absorbance at 450nm in a microplate reader. Calculate the concentration of CD8A in the sample by plotting standard curve. The concentration of the target substance is proportional to the OD450 value. |
