|
Human Hepcidin (Hepc) ELISA Kit - Details |
|
Sensitivity |
5.12pg/ml |
|
Range |
10pg/ml–4000 pg/ml |
|
Sample Type |
use with serum, plasma, culture media, or any biological fluids |
|
Detection Method |
Quantitative Detection |
|
Storage |
2-8℃ |
|
Principle |
The quantitative detection of Human Hepc antigen is based on the ELISA (Enzyme-LinkedImmunosorbent Assay) technique. The microplate wells are pre-coated with an antibody specific to Human Hepc. After incubation, the corresponding antigen in the sample or standards will bind to the immobilized antibody. After washing the microplate wells to remove all unbound sample material, horseradish peroxidase (HRP) labeled antibody conjugate is added, which binds to the antigen, forming antibody-antigen-enzyme-labeled antibody complex upon incubation. In a second wash step, all unbound material is removed. The immune complex formed by the bound conjugate is visualized by adding
Tetramethylbenzidine (TMB) substrate, which gives a blue color reaction in wells containing the complex. A stop solution containing acid terminates the reaction, producing a yellow end-point coloration proportional to the amount of specific antigen present in the sample. Absorbance is measured at 450 nm using an ELISA microwell plate reader. Analysis is completed by comparing the OD values of samples to the standard curve. |
|
Human Hepcidin (Hepc) ELISA Kit - Details |
|
Sensitivity |
3.12pg/mL |
|
Range |
7.81-500pg/mL |
|
Sample Type |
use with serum, plasma, culture media, or any biological fluids |
|
Detection Method |
Sandwich-ELISA |
|
Storage |
2-8℃ |
|
Principle |
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human Hepc. Samples (or Standards) are added to the micro ELISA plate wells and
combined with the specific antibody. Then a biotinylated detection antibody specific for Human Hepc and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free
components are washed away. The substrate solution is added to each well. Only those wells that contain Human Hepc, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm. The OD value is proportional to the concentration of
Human Hepc. You can calculate the concentration of Human Hepc in the samples by comparing the OD of the samples to the standard curve |
