| Human BDNF(Brain Derived Neurotrophic Factor) ELISA Kit - Details | |
| Uniprot ID | P23560 |
| Alternative Names | ANON2, BULN2 |
| Detection Method | Sandwich |
| Standard | 2000pg/mL |
| Sample type | Serum, Plasma, Tissue homogenate and Other biological samples；Sample Volume=100μL |
| Reaction time | 3.5H |
| Research Area | Enzyme & Kinase |
| Test Principle | |
| This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human BDNF. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human BDNF and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human BDNF, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Human BDNF. You can calculate the concentration of Human BDNF in the samples by comparing the OD of the samples to the standard curve. | |
Human BDNF(Brain Derived Neurotrophic Factor) ELISA Kit
| Catalogue No | BSEK-156H |
| Species | Human |
| Sensitivity | 18.75 pg/mL |
| Range | 31.25-2000pg/mL |
| Size | 96 T | 48 T |
| Price | POR |
As the OD values of the standard curve may vary according to the conditions of the actual assay performance (e.g. operator, pipetting technique, washing technique or temperature effects), the operator should establish a standard curve for each test. Typical standard curve and data is provided below for reference only.
