Enzyme immunoassay for the quantitative detection of Rat IL-8 antigen in serum, plasma, culture media, or any biological fluid. Our Rat Interleukin 8 (IL-8)ELISA Kit is to assay IL-8 levels in Rat serum, plasma, culture media or any biological fluid.
| Rat Interleukin 8 (IL-8) ELISA Kit | |
| Sensitivity | 2.52ng/L |
| Range | 5ng/L →1500ng/L |
| Detection Method | Sandwich |
| Assay length | 1-5Hours |
| Detection Wavelength | 450nm |
| Research Area | Immunology |
| Storage | stored at 4℃ upon receipt while the others should be at -20℃ |
| Test Principle | The kit is a sandwich enzyme immunoassay for in vitro quantitative measurement of this index in serum, plasma,tissue homogenates,cell lysates,cell culture supernates and other biological fluids. |
| Rat Interleukin 8 (IL-8) ELISA Kit | |
| Sensitivity | 15pg/ml |
| Range | 40pg/ml-1800pg/ml |
| Standard | 2250pg/ml |
| Wavelength | 450nm |
| Detection Method | Sandwich-ELISA |
| Sample Volume | 10ul |
| Sample type | serum, plasma, culture media, or any biological fluids. |
| Storage | 2-8℃ |
| Test Principle | This ELISA kit uses Sandwich-ELISA as the method. The Micro Elisa strip plate provided in this kit has been pre-coated with an antibody specific to IL-8 Standards or samples are added to the appropriate Micro Elisa strip plate wells and combined to the specific antibody. Then a Horseradish Peroxidase (HRP)-conjugated antibody specific for IL-8 is added to each Micro Elisa strip plate well and incubated. Free components are washed away. The TMB substrate solution is added to each well. Only those wells that contain IL-8 and HRP conjugated IL-8 antibody will appear blue in color and then turn yellow after the addition of the stop solution. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm. The OD value is proportional to the concentration of IL-8 You can calculate the concentration of IL-8 in the samples by comparing the OD of the samples to the standard curve. |