Mitochondrial Tracker (Green) (mitochondrial green fluorescent probe) is a mitochondrial green fluorescent dye that can stain and mark mitochondria at the nanomolar level. The dye has plasma membrane permeability and accumulates on the mitochondrial membrane to show bright fluorescence. The localization of the dye in mitochondria is independent of the mitochondrial membrane potential. The signal-to-noise ratio of stained fixed cells is not ideal. After cell fixation and permeabilization , the fluorescent signal will be weakened or lost, so we recommend that it is only used to stain live cells.

Reagent Preparation

Preparation of stock solution

Prepare 200 μM Mitochondrial Tracker (Green) stock solution: Take a 50 ug tube of Mitochondrial Tracker (Green) dye, add 372 μL of anhydrous DMSO or DMF, and vortex to mix thoroughly. This stock solution is stable at-20°C for 6 months.

The optimal dye concentration and incubation time vary with cell types. We recommend a working concentration of 20-200 nM for Mitochondrial Tracker (Green).It is easy to stain other cell structures.