BCA Protein Assay Kit (Low Concentrations)

Catalouge No BSAK-055
Range 0.39μg/ml - 50μg/ml
Reactivity All
Applications Protein quantification
Size 500 Assays
Price POR
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SKU: BSAK-055 Category:
BCA Protein Assay Kit (Low Concentrations) - Details
Description Detection and Quantification of Protein Concentration.
Detection Method Colorimetric
Components Reagent A: 25 ml x 3 Reagent B: 1.5 ml x 1 Standard: 50 μg x 5
Storage/Stability Shipped and store at 4°C for 1 year.
Sample Types All
Platform Microplate reader 562 nm
Species Reactivity Key E- ELISA, WB - Western blot, IH - Immunohistochemistry, IF - Immunofluorescence, FC - Flow cytometry, IC - Immunocytochemistry, IP - Immunoprecipitation, ChIP - Chromatin Immunoprecipitation, EMSA - Electrophoretic Mobility Shift Assay, BL - Blocking, SE - Sandwich ELISA, CBE - Cell-based ELISA, RNAi - RNA interference
Application
Protein quantification is often required before precessing protein samples for analysis. The Bicinchoninic Acid (BCA) Protein Assay is highly sensitive colorimetric assay that is not affected by chemicals in the sample. The BCA Protein Assay primarily reduces Cu2+ to Cu1+ by proteins in an alkaline environment followed by highly sensitive and selective colorimetric detection of BCA/copper complex. It is water-soluble and strongly absorbs light at 562nm in a linear fashion with increasing protein concentration.

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