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Na+/K+-ATPase α1 Polyclonal Antibody - Details |
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Molecular Weight |
112896Da |
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Purification |
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
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Immunogen |
The antiserum was produced against synthesized peptide derived from human ATPase. AA range:5-54 |
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Gene Name |
ATP1A1 |
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Dilution |
Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/10000. Not yet tested in other applications. |
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Protein Name |
Sodium/potassium-transporting ATPase subunit alpha-1 |
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Human Gene Id |
476 |
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Human Swiss Prot No |
P05023 |
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Mouse Gene Id |
11928 |
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Mouse Swiss Prot No |
Q8VDN2 |
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Rat Gene Id |
24211 |
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Rat Swiss Prot No |
P06685 |
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Specificity |
Na+/K+-ATPase α1 Polyclonal Antibody detects endogenous levels of Na+/K+-ATPase α1 protein. |
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Formulation |
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide. |
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Concentration |
1 mg/ml |
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Storage Stability |
-20°C/1 year |
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Other Name |
ATP1A1; Sodium/potassium-transporting ATPase subunit alpha-1; Na(+)/K(+) ATPase alpha-1 subunit; Sodium pump subunit alpha-1 |
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Observed Band(KD) |
112 |
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Subcellular Location |
Basolateral cell membrane ; Multi-pass membrane protein . Cell membrane, sarcolemma ; Multi-pass membrane protein . Cell projection, axon . Melanosome . Identified by mass spectrometry in melanosome fractions from stage I to stage IV. . |
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Function |
catalytic activity:ATP + H(2)O + Na(+)(In) + K(+)(Out) = ADP + phosphate + Na(+)(Out) + K(+)(In).,function:This is the catalytic component of the active enzyme, which catalyzes the hydrolysis of ATP coupled with the exchange of sodium and potassium ions across the plasma membrane. This action creates the electrochemical gradient of sodium and potassium ions, providing the energy for active transport of various nutrients.,PTM:Phosphorylation on Tyr-10 modulates pumping activity.,similarity:Belongs to the cation transport ATPase (P-type) family.,similarity:Belongs to the cation transport ATPase (P-type) family. Type IIC subfamily.,subcellular location:Identified by mass spectrometry in melanosome fractions from stage I to stage IV.,subunit:Composed of three subunits: alpha (catalytic), beta and gamma. Binds the HLA class II histocompatibility antigen, DR1 |
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Background |
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ATPase Na+/K+ transporting subunit alpha 1(ATP1A1) Homo sapiens The protein encoded by this gene belongs to the family of P-type cation transport ATPases, and to the subfamily of Na+/K+ -ATPases. Na+/K+ -ATPase is an integral membrane protein responsible for establishing and maintaining the electrochemical gradients of Na and K ions across the plasma membrane. These gradients are essential for osmoregulation, for sodium-coupled transport of a variety of organic and inorganic molecules, and for electrical excitability of nerve and muscle. This enzyme is composed of two subunits, a large catalytic subunit (alpha) and a smaller glycoprotein subunit (beta). The catalytic subunit of Na+/K+ -ATPase is encoded by multiple genes. This gene encodes an alpha 1 subunit. Multiple transcript variants encoding different isoforms have been found for this gene. |
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