Human SIRT1 quantitates human SIRT1 in serum, plasma, supernatant. The assay will exclusively recognize both natural and recombinant human SIRT1.
| Human SIRT1 ELISA Kit- Details | |
| Sample type/volume | Serum 50 µL | Supernatant 50 µL | Plasma 50 µL |
| Protein name | SIRT1 |
| Hands-on time | 1 hr 20 min |
| Time-to-result | 4 hr 45 min |
| Interassay CV | <12% |
| Intraassay CV | <10% |
| Storage | Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C |
| Assay kit format | Sandwich ELISA Kit |
| Detection Method | Biotin |
| Assay kit format | Sandwich ELISA Kit |
| Target information | |
| NAD-dependent protein deacetylase sirtuin-1 (SIRT1) links transcriptional regulation directly to intracellular energetics and participates in the coordination of several separated cellular functions such as cell cycle, repsonse to DNA damage, metabolism, apoptosis, and autophagy. SIRT1 can modulate chromatin function through deacetylation of histones and can promote alterations in the methylation of histone and DNA, leading to transcriptional repression. It is essential in skeletal muscle cell differentiation and in response to low nutrients mediates the inhibitory effect on skeletal myoblast differentiation which also involves 5'-AMP-activated protein kinase (AMPK) and nicotinamide phosphoribosyltransferase (NAMPT). | |
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