RPMI 1640 With 1mM Sodium pyruvate, 2mM L-Glutamine, 4.5 gms Glucose per liter, 10mM HEPES buffer Without Sodium bicarbonate.
Application: Human normal and neoplastic leukocytes can be cultured in this medium.
| Ingredients | MG/L |
| INORGANIC SALTS | |
| Ca (NO3)2 4H2O | 100.00 |
| Magnesium sulphate anhydrous | 48.84 |
| Potassium chloride | 400.00 |
| Sodium chloride | 6000.00 |
| Sodium dihydrogen phosphate monohydrate | 800.00 |
| AMINO ACIDS | |
| Glycine | 10.00 |
| L-Arginine | 241.00 |
| L-Asparagine H2O | 50.82 |
| L-Aspartic Acid | 20.00 |
| L-Cystine 2HCl | 65.00 |
| L- Glutamine | 300.00 |
| L-Glutamic Acid | 20.00 |
| L-Histidine HCl H2O | 20.96 |
| L-Hydroxy-L-Proline | 20.00 |
| L-Isoleucine | 50.00 |
| L-Leucine | 50.00 |
| L-Lysine HCl | 40.00 |
| L-Methionine | 15.00 |
| L-Phenylalanine | 15.00 |
| L-Proline | 20.00 |
| L-Serine | 30.00 |
| L-Threonine | 20.00 |
| L-Tryptophan | 5.00 |
| L-Tyrosine 2 Na 2H2O | 29.00 |
| L-Valine | 20.00 |
| VITAMINS | |
| p-Amino Benzoic Acid | 1.00 |
| D-Biotin | 0.20 |
| Choline Chloride | 3.00 |
| D-Calcium Pantothenate | 0.25 |
| Folic Acid | 1.00 |
| i-Inositol | 35.00 |
| Nicotinamide | 1.00 |
| Pyridoxal hydrochloride | 1.00 |
| Thiamine hydrochloride | 1.00 |
| Riboflavin | 0.20 |
| Thiamine HCl | 1.00 |
| Vitamin B12 | 0.005 |
| OTHERS | |
| D-Glucose | 4500.00 |
| HEPES | 2383.00 |
| L-Glutathione Reduced | 1.00 |
| Phenol Red Sodium Salt | 3.80 |