Bradford Reagent is composed of Coomassie Brilliant Blue G-250 dye, ethanol, and phosphoric acid, formulated to produce a stable, ready-to-use solution. The dye binds primarily to arginine and, to a lesser extent, to histidine, lysine, and other basic and aromatic amino acids, causing a shift in the absorbance maximum from 465 nm to 595 nm. This shift in absorbance correlates with the protein concentration, allowing for accurate quantification by measuring the absorbance at 595 nm using a spectrophotometer or plate reader.

Applications

Bradford Reagent is versatile and suitable for a range of applications:

• Protein Quantification: Ideal for determining protein concentrations in cell lysates, tissue homogenates, and purified protein solutions.
• Enzyme Assays: Useful in preparing samples for enzymatic activity measurements by normalizing protein concentrations.
• Western Blotting: Suitable for normalizing protein amounts in samples prior to gel electrophoresis

Composition

The Bradford Reagent is formulated with Coomassie Brilliant Blue G-250 dye, ethanol, and phosphoric acid. It is supplied as a ready-to-use solution, eliminating the need for preparation or dilution.

Storage Conditions

Store the Bradford Reagent at 4°C. Protect from light to prevent degradation of the dye. Ensure the reagent is tightly sealed to prevent contamination and evaporation. The reagent is stable for at least one year when stored as recommended.