| Alternative Names | Nrf2 (phospho S40); p-Nrf2 (phospho S40); Nuclear factor-E2 related factor2; HEBP1; NF E2 related factor 2; NFE2 related factor 2; NFE2L2; Nrf 2; Nuclear factor erythroid 2 related factor 2; Nuclear factor erythroid derived 2 like 2; NF2L2_HUMAN. |
| Antibody type | Recombinant |
| Swiss-Prot No | Q16236 |
| Storage/Stability | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| Immunogen | KLH conjugated Synthesised phosphopeptide derived from human Nrf2 around the phosphorylation site of Ser40 |
| Gene ID | 4780 |
| Subcellular Location | Cytoplasm, cytosol. Nucleus. Note=Cytosolic under unstressed conditions, translocates into the nucleus upon induction by electrophilic agents. |
| Isotype | IgG |
| Immunogen range | DF(p-S)QR aa |
| Molecular Weight | 68 kDa |
| Dilution | 1ug/Test |
| Primary Antibody (green line) | Rabbit Anti-phospho-Nrf2 (Ser40) antibody |
| Secondary Antibody | Goat anti-rabbit IgG-FITC |
| Blank control | THP-1. |
Protocol
The cells were fixed with 4% PFA (10min at room temperature)and then permeabilized with 90% ice-cold methanol for 20 min at -20℃.The cells were then incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.
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