HT Media supplement is a cocktail hypoxanthine and thymidine qualified for use in hybridoma selection and cell culture applications. HT Media Supplement (50×) Hybri-Max^™ has been used as a supplement in INS-1 β-cells co-culture medium.[1] It is suitable for use as medium for hybridoma cells[2] and as Dulbecco′s Modified Eagle Medium (DMEM) for HeLa S3 and MTH1-KO cells.